Journal: EMBO Reports
Article Title: miR-708-5p is elevated in bipolar patients and can induce mood disorder-associated behavior in mice
doi: 10.1038/s44319-025-00410-y
Figure Lengend Snippet: ( A ) Schematic representation of the workflow to obtain PBMCs samples from the FOR2107 cohort (see also Methods and Protocols section). Subjects underwent psychiatric and cognitive evaluation. After the data was collected, blood was withdrawn and PBMCs prepared followed by RNA extraction and downstream gene expression analysis. Samples were collected from psychiatrically healthy female subjects (CTL), or psychiatrically healthy female subjects with genetic predisposition for mood disorder (genetic risk – GR) or that suffered from childhood maltreatment (environmental risk – ER) (Vulnerability samples). ( B ) Volcano plot depicting differentially expressed miRNAs based on small-RNA sequencing from RNA isolated from PBMCs of CTL ( n = 18) vs ER ( n = 18) subjects. Up- (blue) and downregulated (red) miRNAs are labeled. Statistics: differential expression analysis (limma/voom with correction for two technical batches). ( C ) Table showing the top 15 differentially expressed miRNAs from CTL vs ER subjects, ordered from smallest to largest p -value. miRNA-708-5p is highlighted in blue. Statistics: differential expression analysis (limma/voom with correction for two technical batches). ( D ) Volcano plot depicting differentially expressed miRNAs based on small-RNA sequencing from RNA isolated from PBMCs of CTL ( n = 18) vs GR ( n = 14) subjects. Up- (blue) and downregulated (red) miRNAs are labeled. Statistics: differential expression analysis (limma/voom with correction for two technical batches). ( E ) Table showing the top 15 differentially expressed miRNAs from CTL vs GR subjects, ordered from smallest to largest p -value. miRNA-708-5p is highlighted in blue. Statistics: differential expression analysis (limma/voom with correction for two technical batches). ( F ) miR-708-5p qPCR analysis of total RNA isolated from PBMCs of CTL ( n = 18), ER ( n = 16), and GR ( n = 18) subjects. One-way ANOVA with Holm-Sidak’s multiple comparisons test, CTL vs ER, ** P = 0.0064; CTL vs GR, *** P = 0.0006; ER vs GR, ns. Data are presented as violin plots with median, quartiles and data points. See also: Fig. . .
Article Snippet: QuantiGene ViewRNA miRNA Cell Assay Kit , Thermo Fisher , QVCM0001.
Techniques: RNA Extraction, Gene Expression, RNA Sequencing, Isolation, Labeling, Quantitative Proteomics
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